TIRF

The ImagoSeine platform has a TIRF illumination on an AxioObserver Z1 (Zeiss) microscope. This is the same microscope that is used for PALM super-resolution imaging. The TIRF illumination is done through the objective. Two objectives are available for this illumination: a 100x objective, ON 1.46 and a 100x objective, ON 1.56 (using a special immersion oil). 4 lasers can be used on this system (405, 488, 561, 642nm). The detection of the fluorescence signal is done on an EMCCD iXon 897 camera (Andor, QE 90%).

Go to top page