Spinning Disk Confocal

CSU-X1 thermostatted with FRAP module
Fluorescence optical sectionning observation, simultaneous detection of two colours from 400nm to 750nm, transmission light, Nomarski Differential Interferential Contrast (DIC).
Fully motorized stand and piezo stage for automated time-lapse, multi-position and mosaic acquisition. Incubator controlling temperature from 25 to 40°C and CO2 supply.
Module allowing to photoblanket or photoactivate a precise area to study the dynamics (FRAP experiment, photoactivation and photoconversion).

- Inverted and motorized stand Axio Observer.Z1 (Zeiss)
- Acquisition of Normarsky transmitted light and contrast (DIC) images
- Spinning disk head CSU-X1 (Yokogawa)
- 4 lasers 405nm, 488nm, 561nm, 640nm to observe fluorescence
- FRAP module and 2 lasers 405nm, 476nm for dynamic studies
- associated emission filter
- 2 sCMOS PRIME 95 camera (Photometrics)
- MetaMorph acquisition software
- Temperature and CO2 controlled cabinet (Life Imaging Service)

CSU-X1 at room temperature
Fluorescence optical sectionning observation, simultaneous detection of two colours from 400nm to 750nm, transmission light, Nomarski Differential Interferential Contrast (DIC).
Fully motorized stand and piezo stage for automated time-lapse, multi-position and mosaic acquisition.
Automated 3D reconstruction, shading correction and mosaic alignment.
Reserved for samples observed at room temperature from 16°C to 21°C.

- Inverted and motorized stand Axio Observer.Z1 (Zeiss)
- Acquisition of Normarsky transmitted light and contrast (DIC) images
- Spinning disk head CSU-X1 (Yokogawa)
- 4 lasers 405nm, 488nm, 561nm, 640nm to observe fluorescence
- 2 ORCA Fusion camera (Hamamatsu)
- ZEN 2 acquisition software

CSU-W1 thermostat-controlled wide field of view
Fluorescence optical sectionning observation, simultaneous detection of two colours from 400nm to 750nm, transmission light, Nomarski Differential Interferential Contrast (DIC).
Fully motorized stand and piezo stage for automated time-lapse, multi-position and mosaic acquisition. Built-in Autofocus system to eliminate Z-drift. Incubator controlling temperature from 25 to 40°C and CO2 supply.

- Inverted and motorized microscope DMI8 (Leica)
- Autofocus hardware (Leica AFC)
- Acquisition of Normarsky transmitted light and contrast (DIC) images
- Wide field spinning disk head CSU-W1 (Yokogawa - Andor)
- 4 lasers 405nm, 488nm, 561nm, 640nm to observe fluorescence
- associated emission filter
- 2 Orca-Flash 4 V2+ sCMOS cameras (Hamamatsu)
- MetaMorph acquisition software

Go to top page