Principle of widefield

Widefield microscopy is a simple technique which induces a low photobleaching rate compared to confocal microscopy. It suits low magnification or long-time observation of thin samples. You can observe colored samples thanks to color cameras, transparent sample thanks to phase contrast and interferential contrast and observe samples in fluorescence. It is less adapted to thick samples and morphology studies in 3D because of the blur due to the observation planes superposition on the image.
After acquisition, deconvolvution by numerical methods can increase the image resolution and contrast.

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